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Human CD3 epsilon Protein, His Tag (MALS verified)

分子别名(Synonym)

FLJ18683,T3E,TCRE,CD3E,CD3-epsilon

表达区间及表达系统(Source)

Human CD3 epsilon, His Tag (CDE-H5223) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).

Predicted N-terminus: Asp 23

Request for sequence

蛋白结构(Molecular Characterization)

Online(Asp 23 - Asp 126) P07766-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 16.9 kDa. As a result of glycosylation, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 24 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) SDS-PAGE gel

Human CD3 epsilon, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) MALS images

The purity of Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) was more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse Anti Human CD3 at 2 μg/mL (100 μL/well) can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Anti-Human CD3 mAb, mouse IgG1 (Clone # SP34-2) on AMC Biosensor, can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with an affinity constant of 0.341 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Bispecific T-cell Engager (CD3 X BCMA) on AHC Biosensor via DMF Filed Human BCMA, Fc Tag (Cat. No. BC7-H5254), can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with an affinity constant of 0.147 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

文献引用(Citations)

背景(Background)

CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

 

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