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Biotinylated Human CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

分子别名(Synonym)

CD3 epsilon & CD3 gamma,CD3E & CD3G

表达区间及表达系统(Source)

Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (CDG-H82W3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1(CD3E) & AAI13831(CD3G)).

Predicted N-terminus: Asp 23 (CD3E)

Request for sequence

蛋白结构(Molecular Characterization)

Online(Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)) NP_000724.1 (CD3E) & AAI13831 (CD3G)

Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 18.6 kDa (CD3E) and 14.7 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 22 kDa and 24-30 kDa due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) SDS-PAGE gel

Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) MALS images

The purity of Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) was more than 90% and the molecular weight of this protein is around 35-52kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse Anti-Human CD3 (SP34-2) at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) with a linear range of 0.016-0.25 μg/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized UCHT1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) with a linear range of 0.5-31 ng/mL (QC tested).

Protocol

背景(Background)

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

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