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PE-Labeled Human Glypican 3 / GPC3 Protein, His Tag (Site-specific conjugation)

分子别名(Synonym)

GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1

表达区间及表达系统(Source)

PE-Labeled Human Glypican 3, His Tag (GP3-HP2E3) is produced via site-specific conjugation of PE to Human Glypican 3, His Tag under optimal conditions with a proprietary technology. PE-Labeled Human Glypican 3, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).

Predicted N-terminus: Gln 25

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gln 25 - His 559) P51654-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 64.5 kDa.

Conjugate

PE

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 575 nm

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.

活性(Bioactivity)-FACS

Biotinylated Human FACS

5e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human Glypican 3, His Tag (Cat. No. GP3-HP2E3) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

 

前沿进展

 
 
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Glypican 3靶点信息
英文全称:Glypican-3
中文全称:磷脂酰肌醇聚糖3
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:10详情
最高研发阶段:临床二期
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