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Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)

For research use only.

ID Components Size
RAS022-C01 Pre-coated Human ACE2 Microplate 1 plate
RAS022-C02 Positive Control 100 μL
RAS022-C03 Negative Control 100 μL
RAS022-C04 HRP-SARS-CoV-2 Spike RBD 10 μg
RAS022-C05 10xWashing Buffer 50 mL
RAS022-C06 Dilution Buffer 50 mL
RAS022-C07 Substrate Solution 12 mL
RAS022-C08 Stop Solution 7 mL

Background/背景

The newly identified 2019 novel coronavirus (SARS-CoV-2) has posed a serious threat to human health, and it spreads quickly. It is helpf μL to develop the Anti SARS-CoV-2 neutralizing antibody titer serologic assay kit to test the protective neutralizing antibody level in serum.

Application/应用

This kit is developed to measure the levels of anti-SARS-CoV-2 neutralizing antibody in serum/plasma in vitro, which is reflected by the inhibition of ACE2/Spike RBD interaction.

This kit is for research use only.

Reconstitution/重构方法

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage/存储

The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

It is recommended not to freeze thaw more than 3 times for powder.

Assay Principles/原理

This assay kit is used to measure the levels of Anti-SARS-CoV-2 neutralizing antibody through a competitive ELISA. The microplate in the kit has been pre-coated with Human ACE2 protein. First serum samples, Positive control,Negative Control are added to the wells followed by addition of HRP-SARS-CoV-2 Spike RBD. After incubation, the wells are washed and substrate is added to the wells. The reaction is terminated by the addition of stop solution and the intensity of color is measured at 450 nm. The presence of neutralizing antibodies in samples will compete with ACE2 for HRP-SARS-CoV-2 Spike RBD binding. The intensity of assay signal decrease proportionally to the presence of Anti-SARS-CoV-2 neutralizing antibody.

Your experiment will include 5 simple steps:

a) All reagents were returned to room temperature(20℃-25℃) before use.

b) Make series the tested sample and control with ditlution buffer,HRP-SARS-CoV-2 RBD diluted with dilution buffer.

c) Add the HRP-SARS-CoV-2 RBD ,diluted sample and Control add the plate respectively.

d) Wash the plate and add TMB or other colorimetric HRP substrate.

e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

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