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Human CD3E-coupled Magnetic Beads

ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein> 259pmol (4 μg) CD3E/mg Beads> 259pmol (4 μg) CD3E/mg Beads
Binding Capacity>2265 pmol (35μg) Anti Human CD3/mg beads>2265 pmol (35μg) Anti Human CD3/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL ultrapure water (1 mg beads/mL)5 mL ultrapure water (1 mg beads/mL)

Background/背景

The Human CD3E-coupled Magnetic Beads is produced by coupling biotinylated CD3E protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-CD3E antibody from your sample with high specificity.

Application/应用

This product is intended for immunocapture, biopanning and flow cytometry.

Reconstitution/重构方法

See Certificate of Analysis (CoA) for detailed instruction.

Storage/存储

Upon receipt, please store the beads at -20℃ for 1 year in lyophilized state.

Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

Assay Principles/原理

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

Antibody Purification:

1. Resuspend the lyophilized beads by adding the buffer of choice.

2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.

3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.

4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

 

Typical Data/典型数据

Biotinylated Human ELISA

Immobilized 4.1 μg CD3E/1mg beads can bind the Anti Human CD3 Antibody (Cat. No. 551916) with an EC50 of 0.9022 μg/mL (QC tested).

Protocol

Biotinylated Human SPR

Accelerated stability test. After placing the lyophilized beads at 37℃ for 7 days, binding activity between the Human CD3E-coupled Magnetic Beads (Cat.No. MBS-K026) and anti-CD3 antibody showed little deviation from the unaccelerated sample (%RSD<10%). Data were measured on day 0, 3, 7 respectively.

Protocol

Biotinylated Human ELISA

Freeze-thaw stability test. After different freeze-thaw cycles, binding activity between the Human CD3E-coupled Magnetic Beads (Cat.No. MBS-K026) and anti-CD3 antibody showed little deviation from the unfreeze-thaw sample (%RSD<10%). Three freeze-thaw cycles were performed.

Protocol

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