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Anti-SARS-CoV-2 (B.1.1.7) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)

For research use only.

IDComponentsSize
RAS028-C01Pre-coated Human ACE2 Microplate1 plate
RAS028-C02Positive Control100 μL
RAS028-C03Negative Control100 μL
RAS028-C04HRP-SARS-CoV-2 Spike RBD(B.1.1.7) 15 μg
RAS028-C0510xWashing Buffer 50 mL
RAS028-C06Dilution Buffer50 mL
RAS028-C07Substrate Solution12 mL
RAS028-C08Stop Solution7 mL

Background/背景

Multiple variants of SARS-CoV-2 are circulating globally and posting new challenges to human health. As concerns over the potential impacts of the variants grow, people ask if one possesses protective neutralizing antibodies (NAbs) against the variants after receiving the currently available vaccines; and if the NAb therapeutic agents are equally effective to treat the mutant infection as the wild type. To facilitate the mutant-related research, drug trials and vaccine assessments, a high-throughput assay to measure neutralizing antibodies against the mutants is in urgent need.

Application/应用

This kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (B.1.1.7) neutralizing antibody (Spike RBD) in human serum.

It is for research use only.

Storage/存储

The unopened kit should be stored at 2°C to 8°C. The shelf life is 1 year from the date of receipt. The opened kit should be stored at 2°C to 8°C. The shelf life is 1 month from the date of opening.

Assay Principles/原理

This assay kit is used to measure the levels of Anti-SARS-CoV-2 neutralizing antibody through a competitive ELISA. The microplate in the kit has been pre-coated with Human ACE2 protein. First serum samples, Positive control, Negative Control are added to the wells followed by addition of HRP-SARS-CoV-2 Spike RBD. After incubation, the wells are washed and substrate is added to the wells. The reaction is terminated by the addition of stop solution and the intensity of color is measured at 450 nm. The presence of neutralizing antibodies in samples will compete with ACE2 for HRP-SARS-CoV-2 Spike RBD binding. The intensity of assay signal decrease proportionally to the presence of Anti-SARS-CoV-2 neutralizing antibody.

Your experiment will include 5 simple steps:

a) All reagents were returned to room temperature (20℃-25℃) before use.

b) Make series the tested sample and control with ditlution buffer, HRP-SARS-CoV-2 Spike RBD diluted with dilution buffer.

c) Add the diluted sample, Control and the HRP-SARS-CoV-2 Spike RBD add the plate respectively.

d) Wash the plate and add TMB or other colorimetric HRP substrate. e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

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