亚博yabo官网手机登录网站

搜索
 >  经MALS验证的蛋白>CD3E & CD3G >CDG-R82D3

Biotinylated Rabbit CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)

分子别名(Synonym)

CD3 epsilon & CD3 gamma,CD3E & CD3G

表达区间及表达系统(Source)

Biotinylated Rabbit CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (CDG-R82D3) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Thr 120 (CD3E) & Gln 23 - Asn 111 (CD3G) (Accession # Q9TUF9-1(CD3E) & G1SDA3-1(CD3G)).

Predicted N-terminus: Gln 22 (CD3E) & Gln 23 (CD3G)

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gln 22 - Thr 120 (CD3E) & Gln 23 - Asn 111 (CD3G)) Q9TUF9-1 (CD3E) & G1SDA3-1 (CD3G)

Biotinylated Rabbit CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 18.2 kDa (CD3E) and 15.0 kDa (CD3G). Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 18 kDa, 21 kDa and 23 kDa under reducing (R) condition, and 36 kDa and 40-43 kDa under non-reducing (NR) condition (SDS-PAGE).

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Rabbit CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-R82D3) SDS-PAGE gel

Biotinylated Rabbit CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Biotinylated Rabbit CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-R82D3) MALS images

The purity of Biotinylated Rabbit CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-R82D3) was more than 85% and the molecular weight of this protein is around 32-42 kDa verified by SEC-MALS.

Report

背景(Background)

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

前沿进展

 
 
货号/价格
开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定