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Mouse CD3 epsilon&CD3 gamma Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (MALS verified)

分子别名(Synonym)

CD3 epsilon & CD3 gamma,CD3E & CD3G

表达区间及表达系统(Source)

Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (CDG-M52W2) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P22646-1(CD3E) & P11942-1(CD3G)).

Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)

Request for sequence

蛋白结构(Molecular Characterization)

Online(Asp 23 - Asp 108 (CD3E) & Gln 23 - Ser 116 (CD3G)) P22646-1 (CD3E) & P11942-1 (CD3G)

Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 41.5 kDa (CD3E) and 41.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. As a result of glycosylation, the protein migrates as 48-55 kDa under reducing (R) condition, and 100 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-M52W2) SDS-PAGE gel

Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-M52W2) MALS images

The purity of Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-M52W2) was more than 90% and the molecular weight of this protein is around 80-95 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-M52W2) at 1 μg/mL (100 μL/well) can bind Biotinylated CD3e Monoclonal Antibody (Clone : 145-2C11) with a linear range of 0.2-6 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized CD3E Monoclonal Antibody (Clone : 145-2C11) at 1 μg/mL (100 μL/well) can bind Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-M52W2) with a linear range of 0.01-0.25 μg/mL (Routinely tested).

Protocol

背景(Background)

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

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