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Rat CD3 epsilon&CD3 delta Heterodimer Protein, Llama Fc Tag&Llama Fc Tag (MALS verified)

分子别名(Synonym)

CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta , epsilon

表达区间及表达系统(Source)

Rat CD3E&CD3D Heterodimer Protein, Llama Fc Tag&Llama Fc Tag (CDD-R5257) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Asp 103 (CD3E) & Phe 22 - Gly 106 (CD3D) (Accession # D4A5M2-1(CD3E) & P19377-1(CD3D)).

Predicted N-terminus: Gln 21 (CD3E) & Phe 22 (CD3D)

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gln 21 - Asp 103 (CD3E) & Phe 22 - Gly 106 (CD3D)) D4A5M2-1 (CD3E) & P19377-1 (CD3D)

Rat CD3E&CD3D Heterodimer Protein, Llama Fc Tag&Llama Fc Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 40.8 kDa (CD3E) and 40.9 kDa (CD3D). Subunit CD3E is fused with a llama IgG2b Fc tag at the C-terminus and subunit CD3D is fused with a llama IgG2b Fc tag at the C-terminus. The reducing (R) protein migrates as 55-60 kDa due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Rat CD3E&CD3D Heterodimer Protein, Llama Fc Tag&Llama Fc Tag (Cat. No. CDD-R5257) SDS-PAGE gel

Rat CD3E&CD3D Heterodimer Protein, Llama Fc Tag&Llama Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Rat CD3E&CD3D Heterodimer Protein, Llama Fc Tag&Llama Fc Tag (Cat. No. CDD-R5257) MALS images

The purity of Rat CD3E&CD3D Heterodimer Protein, Llama Fc Tag&Llama Fc Tag(Cat. No. CDD-R5257) was more than 85% and the molecular weight of this protein is around 100-115 kDa verified by SEC-MALS.

Report

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

背景(Background)

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

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