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Human CD3E & CD3D Heterodimer-coupled Magnetic Beads

ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein>1522 pmol (49 μg) CD3E & CD3D/mg Beads>1522 pmol (49 μg) CD3E & CD3D/mg Beads
Binding Capacity>267 pmol (40 μg) antibody/mg beads>267 pmol (40 μg) antibody/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL ultrapure water (1 mg beads/mL)5 mL ultrapure water (1 mg beads/mL)

Background/背景

The biotinylated CD3E & CD3D protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-CD3E & CD3D antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This Human CD3E & CD3D coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.

Application/应用

This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.

Reconstitution/重构方法

See Certificate of Analysis (CoA) for detailed instruction.

Storage/存储

Upon receipt, please store the lyophilized beads at -20℃ for long term storage. The shelf life is 1 year at -20℃ in lyophilized form.

Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

Assay Principles/原理

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

Antibody Purification:

1. Resuspend the lyophilized beads by adding the buffer of choice.

2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.

3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.

4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

 

Typical Data/典型数据

Biotinylated Human ELISA

Add CD3 pre-coupling magnetic beads into each well at a concentration of 1 mg/ml. 0.1 mg beads was added to each well. Then add increasing concentrations of anti-CD3 antibody to different wells. PE-labeled anti-human IgG Fc antibody was used for detection (QC tested).

Protocol

Biotinylated Human SPR

The binding curves between CD3E&D pre-coupling magnetic beads (Cat. No. MBS-K003) after different freeze-thaw cycles and anti-CD3 antibody. 0.1 mg of Beads (1 mg/mL, 100 μL) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/mL-0.039 μg/mL) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).

Protocol

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