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Biotinylated Human IL-2 R beta / CD122 Protein, His,Avitag™

分子别名(Synonym)

IL2RB,RP5-1170K4.6,CD122,P70-75

表达区间及表达系统(Source)

Biotinylated Human IL-2 R beta, His,Avitag (ILB-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # P14784-1).

Predicted N-terminus: Ala 27

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ala 27 - Asp 239) P14784-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 28.2 kDa. The protein migrates as 35-38 kDa and 42-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human IL-2 R beta, His,Avitag (Cat. No. ILB-H82E3) SDS-PAGE gel

Biotinylated Human IL-2 R beta, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human IL-2 R beta, His,Avitag (Cat. No. ILB-H82E3) on SA Biosensor, can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

Biotinylated Human BLI

Loaded Biotinylated Human IL-2 R beta, His,Avitag (Cat. No. ILB-H82E3) on SA Biosensor, can bind Human IL-15, Tag Free (Cat. No. IL5-H4117) with an affinity constant of 16.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to a cytokine called IL-2. The IL-2R is made up of 3 subunits - α (CD25), β (CD122) and γ (CD132) - non-covalently associating. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit. CD122 is also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

 

前沿进展

 
 
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IL-2 R beta靶点信息
英文全称:Interleukin-2 receptor beta chain
中文全称:白细胞介素-2受体β链
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:批准上市
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