Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (CDE-M120a) is recombinantly produced from human 293 cells (HEK293).
Mouse IgG2a / kappa
The cross-reactivity with other species has not been tested yet.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Less than 0.002 EU per μg by the LAL method.
The sterility testing was performed by membrane filtration method described in USP <71> and Eur. Ph. 2.6.1.
This product is supplied and shipped as sterile liquid solution with blue ice, please inquire the shipping cost.
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
For long term storage, the product should be stored at liquid state at 4°C.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- 4°C for 12 months in liquid state;
- The product MUST be stored at 4°C upon receipt.
Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) was more than 95% and the molecular weight of this protein is around 138-158 kDa verified by SEC-MALS.
活性（Bioactivity）-Cell based assay
Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) stimulates proliferation of PBMC. The ED50 for this effect is 0.4077 ng/mL (QC tested).
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.